Scientist, Cell Engineering
Research | South San Francisco, CA | Full Time
Allogene Therapeutics is a biotechnology company with a mission to catalyze the next revolution in cancer treatment through the development of allogeneic chimeric antigen receptor T-cell (CAR T) therapy directed at blood cancers and solid tumors. Founded and led by former Kite Pharma executives who bring unrivaled clinical development acumen in cell therapy, Allogene is well-positioned to further the potential of allogeneic cell therapy for patients.
Allogeneic CAR T therapies are engineered from cells of healthy donors and stored for “off-the-shelf” use in patients. This approach eliminates the need to create personalized therapy from a patient’s own cells, simplifies manufacturing, and reduces the time patients must wait for CAR T treatment. The Allogene portfolio includes 16 pre-clinical T cell therapy assets and UCART19, an allogeneic CAR T therapy currently in Phase 1 development for the treatment of acute lymphoblastic leukemia (ALL). Through its notable partnerships, Allogene leverages pioneering technology platforms, including TALEN® gene editing technology, to progress its portfolio of immuno-oncology therapies. Allogene, with headquarters in San Francisco, California, is a Two River portfolio company formed with one of the largest Series A financings in biotechnology from an investment consortium which includes TPG, Vida Ventures, BellCo Capital, the University of California Office of the Chief Investment Officer, and Pfizer. For more information, please visit www.allogene.com, follow @AllogeneTx on Twitter and LinkedIn.
The candidate will work in the Cell Engineering Research team and contribute to the assessment of gene editing methodologies and the cell and molecular biology experiments within this project.
Responsibilities include, but are not limited to:
- The candidate will perform experiments to validate and improve the technology for gene editing (knock-in and knock-out) of CARs, and analyzing the resulting data.
- Perform all in vitro activities required for the project
- Successfully execute and document laboratory procedures and experiments with great attention to detail
- The candidate must be able to communicate clearly and concisely, both verbally and in the form of written reports, aid in drafting protocols, and provide data for regulatory documentation to support clinical development.
- Other duties as assigned
Position Requirements & Experience:
- Bachelor’s Degree with 8+ years of experience, or Master of Science degree with 3+ years of experience or PhD 0-2 years of experience with focus on cell and molecular biology or molecular genetics. Industry experience preferred.
- Strong background in molecular genetic engineering with experience in design and analysis of genome editing experiments is required.
- Use of ZFN, TALENs and/or CRISPR-Cas9 is required.
- Experience with gene knock-in and knockout gene editing techniques as well and resulting molecular and cellular analysis is required.
- Experience in analysis of gene editing data using methodologies such as NGS, Sanger sequencing is highly preferred.
- Direct expertise with production of lentivirus.
- The candidate must have hands on experience with nucleic acid purification and use, including primer design, PCR and standard cloning.
- Experience with tissue culture of multiple cell lines, including primary cells, primary T cells preferred.
- Experience in molecular cloning techniques including, but not limited to PCR cloning, DNA preparation
- Experience in generation of AAV is preferred
- Ability to perform multi-parameter flow cytometric analyses is preferred
- Strong organizational and record keeping skills
- Self-motivated and willing to accept temporary responsibilities outside of initial job description
- A background in Immunology is preferred.
- Excellent organizational skills and an ability to prioritize effectively to deliver results within reasonably established timelines.
- Ability to work independently and as part of a team.
- Strong interpersonal skills including verbal and written communication are essential in this collaborative work environment.
- Candidates must be authorized to work in the U.S.
As an equal opportunity employer, Allogene Inc. is committed to a diverse workforce. Employment decisions regarding recruitment and selection will be made without discrimination based on race, color, religion, national origin, gender, age, sexual orientation, physical or mental disability, genetic information or characteristic, gender identity and expression, veteran status, or other non-job related characteristics or other prohibited grounds specified in applicable federal, state and local laws. In order to ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Era Veterans' Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact the careers email at www.allogene.com for assistance.
For more information about equal employment opportunity protections, please view the ‘EEO is the Law’ poster.